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smart seq ht kit  (TaKaRa)


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    Structured Review

    TaKaRa smart seq ht kit
    Smart Seq Ht Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 456 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smart+seq+ht+kit/SMART-Seq+HT+Kit/pm42144108-101-5-8
    Average 96 stars, based on 456 article reviews
    smart seq ht kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    RNA Sequencing:

    Article Title: Genome compartments guide protamine replacement and genome stability during spermiogenesis
    Article Snippet: Total RNA was isolated from FACS-sorted spermatids using the NucleoSpin RNA XS Plus kit (TaKaRa) according to the manufacturer’s instructions. .. RNA-seq libraries were generated using the SMART-Seq HT Kit (TaKaRa). .. Libraries were sequenced on an Illumina HiSeq X Ten platform in 150-bp paired-end mode.

    Article Title: Phenotypic and transcriptional changes in human brain pericytes induced by phorbol 12-myristate 13-acetate
    Article Snippet: RNA 153 integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies), and 154 samples with an RNA integrity number (RIN) greater than 9 were used for sequencing. .. 155 RNA-seq libraries were prepared with the SMART-Seq HT Kit (Clontech, Santa Clara, 156 CA, USA) followed by the Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA, 157 USA). .. Sequencing was performed on a NovaSeq X Plus platform (Illumina) to a depth of 158 approximately 30 million paired-end reads (100 bp) per sample.

    Generated:

    Article Title: Genome compartments guide protamine replacement and genome stability during spermiogenesis
    Article Snippet: Total RNA was isolated from FACS-sorted spermatids using the NucleoSpin RNA XS Plus kit (TaKaRa) according to the manufacturer’s instructions. .. RNA-seq libraries were generated using the SMART-Seq HT Kit (TaKaRa). .. Libraries were sequenced on an Illumina HiSeq X Ten platform in 150-bp paired-end mode.

    Article Title: Vancomycin eliminates gut deoxycholic acid, restoring ER proteostasis in ILC2s and relieving colitis.
    Article Snippet: .. Full-length cDNA was generated using a SMART-Seq HT kit (Takara); paired-end DNA libraries were generated and indexed using a Nextera XT Library Prep kit and then a Nextera XT Index kit. .. Barcoded samples were pooled and sequenced with the Illumina NovaSeq TM 6000 sequence platform, generating 2 × 150 bp paired-end reads.

    Construct:

    Article Title: SENP1 facilitates the adaptation of colonic non-lymphoid tissue Treg cells and restrains intestinal inflammation.
    Article Snippet: RNA quality was assessed using the Bioanalyzer 2100 platform (Agilent Technologies). .. Libraries were constructed using the SMART-Seq® HT Kit (Takara Bio USA) and sequenced on an Illumina NovaSeq 6000. ..

    Synthesized:

    Article Title: Biomimetic microfibers for myelin-enhancer screening and neural regeneration
    Article Snippet: Total RNA was isolated from rat primary oligodendrocytes or iPSC-derived OLs using RNeasy mini kits (Qiagen). .. Full-length cDNA was synthesized from total RNA using the SMART-Seq HT Kit (Takara Bio, Japan). .. Illumina sequencing libraries were then prepared using the Nextera XT DNA Library Preparation Kit (Illumina).

    Amplification:

    Article Title: An SPP1-SOCS1 pathway constrains interferon responses in tumor-associated macrophages and shapes an immunosuppressive tumor microenvironment.
    Article Snippet: .. The T7 DNA templates were amplified following the instructions of SMART-Seq HT Kit (Takara; 634436), and the PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN; 28704) according to the manufacturer’s instructions. .. The mRNA fragments were then transcribed in vitro using the mMESSAGE mMACHINETM T7 Transcription Kit according to the manufacturer’s protocol (Thermo Scientific, AM1344).

    Polymerase Chain Reaction:

    Article Title: An SPP1-SOCS1 pathway constrains interferon responses in tumor-associated macrophages and shapes an immunosuppressive tumor microenvironment.
    Article Snippet: .. The T7 DNA templates were amplified following the instructions of SMART-Seq HT Kit (Takara; 634436), and the PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN; 28704) according to the manufacturer’s instructions. .. The mRNA fragments were then transcribed in vitro using the mMESSAGE mMACHINETM T7 Transcription Kit according to the manufacturer’s protocol (Thermo Scientific, AM1344).

    Purification:

    Article Title: An SPP1-SOCS1 pathway constrains interferon responses in tumor-associated macrophages and shapes an immunosuppressive tumor microenvironment.
    Article Snippet: .. The T7 DNA templates were amplified following the instructions of SMART-Seq HT Kit (Takara; 634436), and the PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN; 28704) according to the manufacturer’s instructions. .. The mRNA fragments were then transcribed in vitro using the mMESSAGE mMACHINETM T7 Transcription Kit according to the manufacturer’s protocol (Thermo Scientific, AM1344).

    Gel Extraction:

    Article Title: An SPP1-SOCS1 pathway constrains interferon responses in tumor-associated macrophages and shapes an immunosuppressive tumor microenvironment.
    Article Snippet: .. The T7 DNA templates were amplified following the instructions of SMART-Seq HT Kit (Takara; 634436), and the PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN; 28704) according to the manufacturer’s instructions. .. The mRNA fragments were then transcribed in vitro using the mMESSAGE mMACHINETM T7 Transcription Kit according to the manufacturer’s protocol (Thermo Scientific, AM1344).



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